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KMID : 0613820030130060856
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Journal of Life Science
2003 Volume.13 No. 6 p.856 ~ p.864
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Phylogenetic analysis of procaryote by uridylate kinase
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Lee Dong-Geun
Kim Cheol-Min
Kim Sang-Jin
Ha Bae-Jin
Ha Jong-Myuong
Lee Sang-Hyun
Lee Jae-Hwa
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Abstract
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The 16S rRNA gene is the most common gene in the phylogenetic analysis of procaryotes. However very high conservative of 16S rRNA has limitation in the discrimination of highly related organisms, hence other molecule was applied in this study and the result was compared with that of 16S rRNA. Three COGs (Clusters of Orthologous of protein) were only detected in 42 procaryotes ; transcription elongation factor (COG0195), bacterial DNA primase (COG0358) and uridylate kinase (COG0528). Uridylate kinase gene was selected because of the similarity and one single copy number in each genome. Bacteria, belong to same genus, and Archaebacteria were same position with high bootstrap value in phylogenetic tree like the tree of 16S rRNA. However, alpha and epsilon Proteobcteria showed different position and Spirochaetales of Eubacteria was grouped together with Archaebacteria unlike the result of 16S rRNA. Uridylate kinase may compensate the problem of very high conservative of 16S rRNA gene and it would help to access more accurate discrimination and phylogenetic analysis of bacteria.
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KEYWORD
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Uridylate kinase, 16S rRNA, COG (Clusters of Orthologous of protein), phylogeny
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